Local coupling limits desynchrony in the absence of LD cycles.

(A) Schematic depicting the experimental conditions used. Seedlings were grown for 4 d under LL and then imaged, also under LL (LL-to-LL), so that seedlings have never seen an LD cycle. The white triangle represents the beginning of imaging. (B) Quantification of phase coherence by time evolution of the Kuramoto order parameter, R, for simulated GI expression. Solid lines indicate the mean and the shaded region one standard deviation of the mean. (C) Representative phase plot of simulated GI expression across longitudinal sections of the hypocotyl and root of a single seedling under the LL-to-LL condition. (D) Quantification of phase coherence by time evolution of the Kuramoto order parameter, R, for GI::LUC expression. Solid lines indicate the mean and the shaded region one standard deviation of the mean. (E) Representative phase plot of GI::LUC expression across longitudinal sections of the hypocotyl and root of a single seedling under the LL-to-LL condition. For LL-to-LL GI::LUC data, N = 3 and n = 30; for GI model simulations, n = 24. N represents the number of independent experiments and n the total number of organs tracked. See S2 File for exact n, test statistics, and percentage rhythmicity in each organ. Underlying data are available from https://gitlab.com/slcu/teamJL/greenwood_etal_2019. a.u., arbitrary unit; GI, GIGANTEA; LD, light-dark; LL, constant light; LUC, LUCIFERASE.