Increased inflammatory infiltration in med23^Δli mice after the chronic administration of CCl₄.

(A) Liver sections from med23^f/f and med23^Δli mice were stained with HE, F4/80, and DAPI respectively. (B) Quantification of F4/80-positive cells from the liver sections of med23^f/f and med23^Δli mice (med23^f/f, n = 5; med23^Δli, n = 7). (C) The mRNA expression of immune cells markers (F4/80, Cd45, and Cd3g) in whole-liver extracts was analyzed by qRT-PCR (n = 7 per group). (D-F) qRT-PCR analysis of the expression of cytokines, chemokines, and their receptors in the liver (n = 7 per group). Data are presented as means ± SEM. *P < 0.05, **P < 0.01. For underlying data, see S1 Data file. Ccl, C-C motif chemokine ligand; CCl₄, carbon tetrachloride; Ccr, C-C motif chemokine receptor; Cxcl, C-X-C motif chemokine ligand; Cxcr, C-X-C motif chemokine receptor; HE, hematoxylin–eosin; Il, interleukin; med23, Mediator complex subunit 23; med23^Δli, liver-specific knockout of Med23; med23^f/f, med23-floxed; qRT-PCR, quantitative real-time PCR; Tnfα, tumor necrosis factor alpha.