Experimental design for testing the effects of prolonged temperature exposure on Cardinium’s CI phenotype.

Color of bars denotes temperature range experienced at that life stage (no bar = 27°C, red = 32–29°C, blue = 20–17°C). This experimental design was modified slightly for temperature shock and vertical transmission assays. In shock assays, temperature shocks did not extend to multiple life stages. Only the control and larval treatments were performed in the vertical transmission experiment.