Confocal microscopic images of JAM1 in IHGE cells infected with P. gingivalis WT or the Δkgp ΔrgpA ΔrgpB mutant.

(A) IHGE cells were infected with P. gingivalis WT or the Δkgp ΔrgpA ΔrgpB mutant at an MOI of 100 for 1.5 h. The cells were then fixed, stained with DAPI (cyan) and anti-JAM1 (yellow), and analyzed by confocal microscopy. Scale bars, 10 μm. (B, C) Schematic illustration (B) and confocal microscopic cross-sectional images (C) of the 3D-tissue model of IHGE cells. Gingival epithelial tissues on coverslips were infected with P. gingivalis WT or the Δkgp ΔrgpA ΔrgpB mutant for 2 h. The tissues were then fixed, stained with anti-JAM1 (white) and Alexa Fluor 568–conjugated phalloidin (magenta), and analyzed by confocal microscopy. Scale bars, 30 μm.