BtpA and BtpB TIR filaments are not coincident with yeast tubulin.

Coronas-Serna, Julia María; Louche, Arthur; Rodríguez-Escudero, María; Roussin, Morgane; Imbert, Paul R. C.; Rodríguez-Escudero, Isabel; Terradot, Laurent; Molina, María; Gorvel, Jean-Pierre; Cid, Víctor J.; Salcedo, Suzana P.

doi:10.1371/journal.ppat.1007979.s002

ppat.1007979.s002.pdf (7.15 MB)

BtpA and BtpB TIR filaments are not coincident with yeast tubulin.

journal contribution

posted on 2020-04-28, 17:46 authored by Julia María Coronas-Serna, Arthur Louche, María Rodríguez-Escudero, Morgane Roussin, Paul R. C. Imbert, Isabel Rodríguez-Escudero, Laurent Terradot, María Molina, Jean-Pierre Gorvel, Víctor J. Cid, Suzana P. Salcedo

Indirect immunofluorescence of YPH499 yeast cells expressing GFP-BtpA-TIR, GFP-BtpB-TIR, and their corresponding E234A mutant versions from pYES2 plasmid derivatives (green). Microtubules are stained using anti-tubulin antibody (red). Nuclei are labelled with DAPI (blue). Scale bars correspond to 5 μm.

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