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Bclaf1 interacts with ISGF3 mainly through STAT2.

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posted on 2019-01-25, 20:28 authored by Chao Qin, Rui Zhang, Yue Lang, Anwen Shao, Aotian Xu, Wenhai Feng, Jun Han, Mengdong Wang, Wanwei He, Cuilian Yu, Jun Tang

(A) GST pulldown analysis of the interaction between His-STAT1/STAT2/IRF9 and GST-Bclaf1 F2. (B) IB analysis of immunoprecipitates of HEK293T cells co-transfected with Flag-tagged Bclaf1, Ha-tagged STAT1 or STAT2/IRF9 expression plasmids. (C) IB analysis of immunoprecipitates of HEK293T cells co-transfected with Flag-tagged Bclaf1, Ha-tagged IRF9 or STAT2/STAT1 expression plasmids. (D) IB analysis of STAT1, STAT2, IRF9 and Flag-Bclaf1 in nuclear immunoprecipitates of a HEp-2-Flag-Bclaf1 cell line transfected with si-control or si-STAT2 followed by PBS or human IFNα (500U/mL) treatment for 3h. (E) IB analysis of Bio-ISRE pull-down STAT1, STAT2, IRF9 and Bclaf1.

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    PLOS Pathogens

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    STATISREBclaf 1Bclaf 1 functionsinterferon responseHSV -1. MechanisticallyPRVtranscription factor 1report Bcl -2Herpes simplex virus type 1IFN-stimulated response elementsinfectionIFN-stimulated gene factor 3alphaherpesvirus Pseudorabies virusgene transcriptionISGF

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