Cell tracing experiment demonstrates GFP-positive M-CSF-cultured myeloid cells in hair follicle-like structures in wounded skin of mice.
Mice received dermal injection of one million GFP-positive, M-CSF-cultured myeloid cells were created excisional dermal wounds by punch biopsy. Mice were euthanized after 4 weeks and skin was collected and immunofluorescent stained for detection of GFP (green). DAPI (blue) was used as a nuclear counterstain. Scale bars in all images were 50 μm.