pone.0262060.s004.pptx (1.98 MB)
Download fileCell tracing experiment demonstrates GFP-positive M-CSF-cultured myeloid cells in hair follicle-like structures in wounded skin of mice.
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posted on 23.06.2022, 20:05 authored by Yunyuan Li, Hatem Alnojeidi, Ruhangiz T. Kilani, Aziz GhaharyMice received dermal injection of one million GFP-positive, M-CSF-cultured myeloid cells were created excisional dermal wounds by punch biopsy. Mice were euthanized after 4 weeks and skin was collected and immunofluorescent stained for detection of GFP (green). DAPI (blue) was used as a nuclear counterstain. Scale bars in all images were 50 μm.
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play important rolesmany cell typeshair follicle regenerationdouble immunofluorescent stainingcell tracing techniquemyeloid cell contributionsinflammatory cells infiltratewhether myeloid cellspositive myeloid cellsderived myeloid cellsk14 antibodies indicateddiv >< pstimulated myeloid cellsdermal wound healingwound healing processmyeloid cellshealing processwound sitewound repairlike cellshematopoietic cellsepithelial cellsendothelial cellscomplex processstimulated cd11bwork highlightsvivo </significant proportionresult showeddermal woundsdamaged tissue
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