Two-dimensional ¹H-¹⁵N HSQC spectrum of E49D and E49A compared with wildtype PRC1-DD.

(a) Ribbon representation of solution structure, in which residues E49, H46 and those residues that interact with it were stressed in ball-and-stick representation. (b) crystal structure of PRC1-DD, in which residues E49, H46 and those residues that interact with it were stressed in ball-and-stick representation. (c) The superposition of two-dimensional ¹H-¹⁵N HSQC spectrum of wildtype(black) and E49D(red), in which residues exhibiting large changes are denoted. (d) The superposition of two-dimensional ¹H-¹⁵N HSQC spectrum of wildtype(black) and E49A(red), in which residues exhibiting large changes are denoted.

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