Text and GitHub link for DAB-quant published on protocols.io at DOI: dx.doi.org/10.17504/protocols.io.dm6gpb578lzp/v1.
journal contribution
posted on 2022-07-20, 17:37 authored by Sneh Patel, Sara Fridovich-Keil, Shauna A. Rasmussen, Judith L. Fridovich-Keil(DOCX)
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source program designedstained tissue pixelspixels deemed tissuediv >< psource digital systemquantifying immunohistochemical stainingfacilitate objective quantitation3 ′- diaminobenzidinetissue slides stainednegative control slidesthreshold distinguishing tissuedab )unstained tissuetissue areanegative controlstest slidesscanned slidesusage instructionsseparate folderssample datapublicly availablepseudocolor imagesprove usefulproblem areasotsu ’large numbersgithub (<enables exclusionempty spacedescribe dabdefined tolerancedab ).color intensitybroad rangeautomatically across
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