Step-by-step protocol to use the FastCloneAssist in local Python Environment.docx.
journal contribution
posted on 2025-03-13, 17:37 authored by Pradip Kumar Singh, Michael S. DonnenbergStep-by-step protocol to use the FastCloneAssist in local Python Environment.docx.
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xlink "> howeverxlink "> fastcloningstep traditional methodsoptimizing primer designli et alfriendly python programdesired vector positiondesired melting temperatureconstructing fusion proteinsrevolutionizing molecular cloningpowerful technique accessiblefailed cloning attemptsefficient pcr amplificationstreamlined python toolminimal user inputpcr cloninginnovative techniqueaccelerates cloningtm ),seamless integrationpresent fastcloneassistparadigm shiftmanipulating genesintroducing fastcloneassistinsert sequencesinsert dnaeliminating laboriousdpni digestioncritical hurdlechimeric cdnas
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