Species-specific targets included in the multiplex-qPCR.
journal contribution
posted on 2024-06-25, 17:55 authored by Jonathan Jacobson, Loraine Fabri, Joshua Osowicki, Shivanthan Shanthikumar, Anna-Maria Costa, Belinda Ortika, Ashleigh Wee-Hee, Michelle Pragassen, Cassandra Gatt, Gena Gonis, Cattram Nguyen, Thomas Rozen, Warwick Teague, Jim Buttery, Vanessa Clifford, Kim Mulholland, Andrew Steer, Sarath Ranganathan, Andrew Daley, Eileen Dunne, Catherine SatzkeSpecies-specific targets included in the multiplex-qPCR.
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untargeted antibiotic therapydetermined clinical sensitivity5 – 231 %) sensitivityperformance determined usingevaluate clinical performanceusing spiked samplesmedian 20 daysstreptococcus pyogenes staphylococcus aureus haemophilus influenzae study gold standardqpcr demonstrated 213enrolling children hospitaliseddiv >< pstreptococcus pneumoniae 90 %) specimens100 %) specificitybacterial culture (<67 %) specimens90 %) cultureimprove bacterial identificationpneumoniae performance comparednegative specimensbacterial cultureprepared samplesmedian agep bacterial pathogenbacterial causeobservational study24 %)serious complicationquantitative pcrqpcr wouldpleural fluidsmolecular assessmentmolecular assaymay allowiqr 17common pathogen9 years6 ).267 ).100 children001 ).
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