S2 Fig -

(A) Inhibition of translation and transcription by means of actinomycin D and cycloheximide led to a decrease in GRK6 protein expression detected in Western blot assay. The main GRK6 band was detected at approx. 70 kDa (molecular weight 66 kDa [22]). Actin β at approx. 40 kDa (molecular weight 43 kDa) was used as reference protein on a stripped membrane. Stimulation with 100 nM PMA, actinomycin D (5 μg/ml) or cycloheximide (20 μg/ml) was carried out in serum starved Jurkat cells for 6 hours as indicated. DMSO was used as vehicle control. This figure is cropped. Full-length blots are represented in S2B and S2C Fig. (B) Uncropped Western blot of GRK6 protein expression after treatment with actinomycin D and cycloheximide as indicated for six hours in serum starved Jurkat cells. For details see caption S2A Fig. Detection of GRK6 protein at approx. 70 kDa. (C) Uncropped Western blot of actin β protein expression after treatment with actinomycin D and cycloheximide as indicated for six hours in serum starved Jurkat cells. For details see caption S2A Fig. After membrane stripping, actin β was used as reference protein.

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