Lipidomic analysis of HFD-sensitive differences depending on p110α.

(A) Heatmap of hepatic lipid quantification in liver samples (n = 6/group). Hierarchical clustering highlights seven different clusters based on the lipid levels. (B) Relative abundance of phosphatidyl ethanolamine (PE) 40:3 and phosphatidyl choline (PC) 36:3 (n = 6/genotype/experimental condition). (C) Relative abundance of PC 32:1 and triglyceride (TG) C51 (n = 6/genotype/experimental condition). (D) Relative abundance of TG C55 and ceramide (Cer) C18:0 (n = 6/genotype/experimental condition). (E) Relative abundance of PE 38:4 and Cer C16:0 (n = 6/genotype/experimental condition). (F) Relative abundance of esterified cholesterol C18 and PE 36:1 (n = 6/genotype/experimental condition). (G) Relative abundance of fatty acid C20:5n-3 and PI 36:1. (H) Differentially expressed genes involved in lipid trafficking, fatty acid metabolism, unsaturated fatty acid metabolism, lipid droplets, cholesterol metabolism, phospholipid metabolism, sphingolipid metabolism, and cannabinoid metabolism between p110α^hep+/+ and p110α^hep−/− in mice fed CTRL diet (yellow) and HFD (grey), respectively. Data information: In all graphs, data are presented as mean ± SEM. ^#P ≤ 0.05 for treatment effect, *P ≤ 0.05 for genotype effect. The numerical values underlying the panels for this figure can be found in S12 Data.

(PDF)