ESR spectra overlay.
journal contribution
posted on 2024-05-17, 17:50 authored by Gennaro Esposito, Yamanappa Hunashal, Mathias Percipalle, Federico Fogolari, Tomas Venit, Ainars Leonchiks, Kristin C. Gunsalus, Fabio Piano, Piergiorgio PercipalleThe ESR spectra of A) TEMPOL alone (black trace), TEMPOL with SARS-CoV-2 Nsp9 + 2NSP90 (red trace), and B) TEMPOL with SARS-CoV-2 Nsp9 + 2NSP23 (green trace), TEMPOL with SARS-CoV-2 Nsp9 (blue trace) superimpose very well, confirming the invariance of the nitroxide dynamic regime in the presence of the proteins and hence the absence of specific tight interactions, consistently with the τc values reported in the main text that were calculated from signal spacings and amplitudes [18–20]. The trace overlay was split into two panels to avoid graphic crowding. The concentration of any species was always 10 μM.
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two epitope pairstetramers resulted plausiblerecent crystal structureprevious characterization suggestingmolecular dynamics simulationsloss perturbation ).efficient antiviral strategynmr spectroscopy usingexperimental nmr evidencenanobody exhibiting essentiallystructural protein nsp9nsp9 relative invariancertc would represent2 stoichiometry suggestedassessing nanobody interactionnanobody stoichiometrynmr resultsnanobody assembliesnsp9 unitsnsp9 tetramernsp9 monomernsp9 complexxlink ">viral grnatranscription complextetramer surfacesubsequent propagationpdb idoligomerization statemapped epitopesliquid stateessential componentdimer contactsdifferent stoichiometriesdifferent distributiondifferent coronavirusesalso analyzed8dqu ),2 non
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