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Divalent cations influence AcrIIA22’s nicking activity.

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journal contribution
posted on 13.10.2021, 17:48 by Kevin J. Forsberg, Danica T. Schmidtke, Rachel Werther, Ruben V. Uribe, Deanna Hausman, Morten O. A. Sommer, Barry L. Stoddard, Brett K. Kaiser, Harmit S. Malik

(A) We present the impact of different divalent cations on AcrIIA22’s nicking activity, which is highest with Mg2+, Mn2+, and Co2+. (B) The OC plasmid product persists through phenol-chloroform extraction following AcrIIA22 treatment, indicating that it directly results from AcrIIA22’s nicking activity. OC, open-circle; SC, supercoiled.