qPCR and Western blot analysis of TsSPc expression at different stages of T. spiralis.

A: qPCR assay of TsSPc transcription levels in diverse T. spiralis phases. Relative transcription level of TsSPc at the 3 d AW stage was evidently higher than those of other worm stages. B: Western blot analysis of TsSPc expression levels at diverse T. spiralis phases. Expression levels of TsSPc protein in crude antigens of diverse T. spiralis phase (ML, IIL, 3 d AW, 6 d AW and NBL) were ascertained by Western blot with 1:100 dilutions of anti-rTsSPc serum. The graph reveals the relative protein expression assayed with densitometry in 3 independent experiments. * P < 0.05 compared to the ML stage.