cSADDs inhibits paraquat mediated signaling to hsp-6 through KGB-1.

A-B. In kgb-1(um3) mutant animals, which are cSADDs deficient, paraquat induced hsp-6 induction is not blocked by elt-2 RNAi. Thus, ROS induced UPR^mt is activated in the absence of functional cSADDs. In contrast, kgb-1(um3) does not prevent inhibition of hsp-6 induction by atfs-1 knockdown, suggesting that it functions downstream of kgb-1 and the cSADDs. Columns represent normalized values plus standard error of the mean (SEM). Numbers in or on columns indicate the number of analyzed animals (n_total = 181). ***: p<0.001, *: p<0.05; Kruskal-Wallis test plus Dunn's Multiple Comparison Test (A). Equal optical settings, scale bar 400 μm. (i): RNAi; L4440: empty vector control; +: wild type allele (B). C. Model: Genes activating the cSADDs (cellular surveillance system) inhibit the paraquat-triggered induction of the UPR^mt.