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siRNA targeting NRF1 mRNA knocks down SPAST

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posted on 04.05.2012 by Brian J. Henson, Wan Zhu, Kelsey Hardaway, Jaime L. Wetzel, Mihaela Stefan, Kathryn M. Albers, Robert D. Nicholls

mRNA expression. SK-N-SH cells were transfected with a pSUPER-NRF1 shRNA expression vector or mock transfected, with qualitative analysis of gene expression including a negative control (GAPDH). The quantitative band intensities for the “siRNA” and “mock” transfection samples were compared and the ratio is listed to the right of the gel images. The GAPDH mRNA level is unaffected by siRNA targeting NRF1, whereas the mRNA levels for NRF1 and SPAST are reduced by the siRNA treatment. The experiment was repeated three times with equivalent results to the representative results shown here.