siRNA mediated knockdown of NBS1 decreased the levels of phosphorylated Akt (pAkt Ser-473) and its downstream targets and IR increased the levels of NBS1 and phosphorylated Akt.
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posted on 2013-06-06, 01:02 authored by Jian-Qiu Wang, Jian-Hong Chen, Yen-Chung Chen, Mei-Yu Chen, Chia-Ying Hsieh, Shu-Chun Teng, Kou-Juey WuA. Western blot analysis showed that knockdown of NBS1 decreased the phosphorylated levels of Akt, GSK-3β, and Foxo1/3a). B. Ionizing radiation of two different cell lines (H1299, OCEM-1) increased the levels of NBS1 and phosphorylated Akt. C. Knockdown of NBS1 in H1299 cells abolished the increase in NBS1 and phosphorylated Akt levels under IR.
