scl1 and lmo2 act downstream of hoxd4a to direct formation of the hemangioblast.

All images are of hoxd4a morphants at 13 hpf. In situ hybridization was performed to detect expression of scl1 and lmo2 (A–D), gata1 and hbbe1 (E–H) and fli1 and flk1 (I–L). To test for rescue of gene expression, embryos were co-injected with capped mRNA for either scl1 or fli1 as indicated on the left. Ratios indicate the fraction of embryos showing the presented phenotype. Scale bars equal 100 µm.