Figure_1.tif (847.07 kB)
rAAV replication in MO59K cells and 293 cell treated with wortmannin.
figure
posted on 2013-02-20, 21:42 authored by Young-Kook Choi, Kevin Nash, Barry J. Byrne, Nicholas Muzyczka, Sihong SongHirt DNA was purified two days after viral infection and subjected to Southern blot analysis. All samples were triplicated and hybridized with 32P labeled CMV probe. Replicated forms of rAAV include double-stranded monomer (about 3.4 kb), or dimer (about 6.8 kb), and concatamers DNA (high molecular weight). Notice that treatment of wortmannin reduced rAAV replication in a dose-dependent manner in both MO59K cells (A) and in 293 cells (B).