pSTAT3 and c-fos expression in ARC neurons in leptin-treated mice.

A–D) Representative immunohistochemistry (IHC) images showing pSTAT3 immunoreactivity following ip PBS (saline) or leptin injection. Scale bar, 100 µm. E) Magel2-null mice have fewer pSTAT3 positive cells than control following leptin injection (Bregma −1.28, P<0.001; Bregma −1.64, P<0.005; Bregma −2.12, P>0.5, *compared between genotypes by Student's t-test) but both genotypes have more pSTAT3 positive cells after leptin treatment than after PBS (#, P<0.05, compared between treatments by Student's t-test; n = 8 mice of each genotype). F–I) Representative images of c-fos IHC following ip PBS and leptin injection. Scale bar 100 µm. J) Leptin induced c-fos expression in both Magel2-null and control mice compared to PBS (#P<0.05, compared between treatments by Student's t-test). At both baseline and following leptin treatment, Magel2-null mice had significantly fewer c-fos positive cells compared to controls (Bregma −1.28 ∼18% reduction, Bregma −1.64 ∼20% reduction, Bregma −2.12 ∼35% reduction, *P<0.05, compared between genotypes by Student's t-test). Values in E) and J) are means ± SEM.