Figure_4.tif (143.16 kB)
pET32a constructs for wtPEDF and coPEDF protein expression in E.coli.
figure
posted on 2013-02-20, 22:04 authored by Anzor G. Gvritishvili, Kar Wah Leung, Joyce Tombran-TinkThe figure shows wtPEDF and coPEDF inserts, upstream thioredoxin (Trx) fragment, factor Xα cleavage site, and mRNA folding energy of 3.9 kcal/mol at the Trx start site (−3 to +50) of the fusion proteins.