p53 localizes to the nucleus following infection with RVFV.
A) HSAECs were either mock infected or infected with MP-12 (MOI 3.0). At 24 hours post infection the cells were washed in PBS without Ca and Mg, permeabilized with Triton X-100 and immunostained with anti-p53 primary antibody using Alexa-Fluor 568 secondary antibody and with anti-RVFV N protein antibody using Alexa-Fluor 488 secondary antibody. The nucleus was stained with DAPI. B) HSAECs were mock infected or infected with MP-12 (MOI 3.0) and collected at 24 hours post-infection for the creation of cytoplasmic and nuclear extracts, CE and NE. Western blot analysis was performed with antibodies against lamin, GAPDH, total p53 and RVFV N protein.