nape over-expression modulates lifespan and dauer formation in daf-2 mutants.
figure
posted on 2014-11-25, 02:49 authored by Neale Harrison, Museer A. Lone, Tiffany K. Kaul, Pedro Reis Rodrigues, Ifedayo Victor Ogungbe, Matthew S. Gill(A) nape-1 or nape-2 over-expression has no effect on lifespan of daf-2(e1368) mutants at 20°C. (B) nape-1(OE), but not nape-2(OE), suppresses daf-2(e1368) lifespan at 25°C (p<0.0001). (C) nape-1(OE) and nape-2(OE) have a small but significant suppressive effect on dauer formation in daf-2(e1368) at 25°C (mean+sd, n = 4 replicates). (D) nape-1(OE) and nape-2(OE) enhances dauer recovery in daf-2(e1368). Loss of the fatty acid desaturase fat-4, which eliminates EPEA and AEA, leads to reduced dauer recovery in daf-2(e1368), daf-2(e1368); nape-1(OE) and daf-2(e1368); nape-2(OE) (mean+sd, n = 9 trials from 3 biological replicates ANOVA p<0.0001, pairwise comparisons *p<0.05, **p<0.01, ***p<0.001).
