Fig_6.tif (1.19 MB)

lncRNA-GAS5 affects the cell cycle and apoptosis of HSVSMCs.

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posted on 2015-03-25, 04:23 authored by Li Li, Xiang Li, Erlinda The, Li-Jie Wang, Tian-You Yuan, Shi-Yi Wang, Jing Feng, Jing Wang, Yuan Liu, Ya-Han Wu, Xiu-E Ma, Jin Ge, Ying-Yu Cui, Xiao-Yan Jiang

As Negative regulatory factors of Cyclin Dependent Kinase, p27-kip1and p21-cip1 can delay the process of cell cycle acting as antagonist to Cyclin. Therefore, they were chosen to reflected cell cycle changes indirectly. A: The p27-kip1and p21-cip1 mRNA expression levels were measured by Q-RT-PCR with knockdown of lncRNA-GAS5 in HSVSMCs. Values are mean±SE, N = 3; *, P<0.05. B: The p27-kip1and p21-cip1 mRNA expression level were measured by Q-RT-PCR with overexpression of lncRNA-GAS5 in HSVSMCs. Values are mean±SE, N = 3; *, P<0.05. C: The apoptosis of HSVSMCs were measured by Flow Cytometry using Annexin V-FITC apoptosis kit. The result shows that the HSVSMCs in early apoptosis decreased with overexpression of lncRNA-GAS5. N = 3; *, P<0.05.