orb genetically interacts with aPKC for DV polarity and oocyte nucleus position.

(A) Top: Dorsal appendage morphology: wild type and ventralized classes V2-V4 [73]. V2 is a reduction in the space between dorsal appendages, V3 is a fusion of the dorsal appendages and V4 is the most severe reduction in dorsal appendages. Bottom: Graph shows the percentage of ventralized eggs laid by wild type, orb heterozygotes (343/+), double-heterozygotes between orb, aPKC alleles (ex48, ex55 and k06403), and cdc42⁴, double-heterozygotes between cdc42⁴ and aPKC^k06403 and triple heterozygotes of orb³⁴³, aPKC^k06403 and cdc42⁴. Frequency of DV polarity defects for heterozygous controls not shown in the graph are listed in S1 Table. n = >400 all genotypes. (B) Top: asterisks show mispositioned oocyte nuclei in aPKC^k06403/+; orb^343/mel (left shows a “float” position and right shows a lateral position). Scale bars 50 microns. Bottom: aPKC^k06403 enhances orb mutant defects in oocyte nucleus position. Defects in aPKC^k06403/+ (2%, n = 50), aPKC^k06403/+; orb^343/mel (27%, n = 118) and aPKC^k06403/+; orb^mel/HD, orb³⁴³ (50%, n = 100). Scale bars are 50 microns.