aPKC mRNA localization depends on orb and Orb binds to aPKC 3’UTRs.

(A and B) In wild type egg chambers aPKC mRNA is localized along the anterior lateral cortex of the oocyte (arrowheads). (B) When orb RNAi (64002) is driven by a midstage driver, maternal α-tubulin Gal4 (7062), aPKC mRNA localization is patchy along the oocyte cortex (arrowheads), or is not localized along the cortex (D and E). (F) Orb immunoprecipitation followed by RT-qPCR shows enrichment of orb-RA 3’UTR and multiple species of aPKC 3’UTRs. The enrichment is calculated using ΔΔCT, and orb and aPKC 3’UTRs are significantly enriched compared to the control 3’UTR, RPL32 (n = 3 biological replicates).