Tsc1 mutant proliferating cells undergo massive apoptosis.

(A) Cleaved Caspase-3 antibody staining (in red) of control or Tsc1 mutant eye discs dissected at the indicated developmental points from larvae reared on normal food and NR; quantification of eye discs size (A’), and percentage of apoptotic tissue area with respect to the total area of discs (A”). Scale bars are 250 μm and 100 μm, respectively.