SdLv inhibits Mab-mediated the generation of TNF-α and IL-6 in BMDMs.

(A and B) BMDMs were infected with Mab (MOI = 5) for the indicated time periods (A) Cell lysates was collected and the mRNA expression of Tnf, Il6 and Il1b then measured using semiquantitative RT-PCR analysis. Actb (encoding β-actin) serves as a loading control throughout. (B) Culture supernatant was collected and the generation of TNF-α and IL-6 protein then were measured using ELISA assay. (C) BMDMs were infected with Mab (at MOI = 0.1, 1, 5 or 10) for 18 h. semiquantitative RT-PCR analysis of Tnf and Il6 mRNA. (D—F) BMDMs were stimulated with increasing concentration of SdLv (1 h, 5–200μg/ml), followed by Mab infection (MOI = 5) for 18 h. (D and E) Semi-quantitative RT-PCR (top) or Quantitative RT-PCR analysis (bottom) were assessed to evaluate the mRNA expression of Tnf (for D) and Il6 (for E). (F) Each culture supernatant was collected and the production of TNF-α and IL-6 were measured using ELISA assay. Data are representative of three independent experiments and are presented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student’s t-test), compared with uninfected cells (B and C) or Mab-infected control cells (D—F). U, Untreated; SC, vehicle control (0.01% DMSO).