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Rv3634c from Mycobacterium tuberculosis H37Rv encodes an enzyme with UDP-Gal/Glc and UDP-GalNAc 4-epimerase activities - Fig 7

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posted on 2017-04-12, 18:20 authored by Peehu Pardeshi, K. Krishnamurthy Rao, Petety V. Balaji

HPLC profiles for 1mM UDP-GalNAc (a), 1mM UDP-GlcNAc (b), 100μM mixture of UDP-GalNAc and UDP-GlcNAC (c), and reaction mixture containing 500μM UDP-GalNAc and 500ng of H3634 incubated for 7.5h at 37°C (d).

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    NADN-terminal His-tagged proteinHPLCSer 121UDP-GalNAc 4- epimerase activityORF Rv 3634cGOD-PODCampylobacter jejuni protein glycosylation enzymesLys 150Tyr 146Mycobacterium tuberculosis H 37RvUDP-GalNAc 4- epimerase activitiesMycobacterium tuberculosis H 37Rv encodesRv 3634c

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