Fig 7.tif (1.18 MB)

Nox overexpression activates canonical Wnt signaling, but does not promote differentiation.

Download (1.18 MB)
figure
posted on 02.02.2017 by Benjamin J. Dickson, Mohamed I. Gatie, Danielle M. Spice, Gregory M. Kelly

(A) Dual-luciferase assay of TCF activity in F9 cells transfected with EV and treated with DMSO or RA, or transfected with pcDNA3.1-Nox1 or pcDNA3.1-Nox4. (B) Dab2 expression in F9 cells transfected with EV and treated with DMSO or RA, or transfected with pcDNA3.1-Nox1. (C) Dab2 expression in F9 cells transfected with EV and treated with DMSO or RA, or transfected with pcDNA3.1-Nox4. (D) Immunoblot analysis for DAB2, TROMA1, and OCT4 in F9 cells transfected with EV and treated with DMSO or RA, or transfected with pcDNA3.1-Nox1, pcDNA3.1-Nox4 or both vectors. β-actin was used as a loading control. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. Letters denote groups of significance (P < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.

History

Licence

Exports