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<i>In vitro</i> recycling of 2-Cys Prx by PDI-M.

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posted on 2017-03-31, 18:10 authored by Benjamin Selles, Flavien Zannini, Jérémy Couturier, Jean-Pierre Jacquot, Nicolas Rouhier
<p>The peroxidase activity of <i>A</i>. <i>thaliana</i> 2-Cys Prx was assessed using a coupled assay measuring NADPH oxidation. Results obtained for PDI-M(<i>a°</i>) or PDI-M(<i>a</i>) are represented as percentages relative to the activity obtained with PDI-M fixed at 100%. Measurements were made in triplicate and error bars indicate standard deviation.</p>

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    Fe 2 S 2reduction reactions-170 mVmoduleAtypical protein disulfide isomerasesNADPH thioredoxin reductasepH 7.0.oxidationWCKHCPDI-L 1A Protein disulfide isomerasesnon-physiological substrate NADP-MDHb domains influencemulti-modular redox catalystspoplar PDI isoformsdithiol-disulfide exchange reactionsPDI-L 1aEscherichia coli expressionPDI-APDI-MNADP malate dehydrogenase

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