In vitro culture of isolated antipodal cells.

A-B. Confocal images of just isolated antipodal cells. D-F. Confocal images of antipodal cells after 3–5 hours’ culture. Notice the dotted fluorescence. G-I. Fluorescent microscopic images of antipodal cells after one week culture. J-L. Fluorescent microscopic images of antipodal cells in the culture chamber after one week culture. Notice the condensed fluorescence of nuclei. Bar = 5μm.