Fig 10.tif (1.59 MB)
In silico analysis of transcriptional activity associated with array peptides with significantly altered phosphorylation levels.
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posted on 2018-01-19, 21:09 authored by Unni Gopinathan, Kathrine Røe Redalen, Anne-Marie Trøseid, Peter Kierulf, Petter Brandtzaeg, Anne Hansen Ree, Jens Petter Berg, Reidun ØvstebøThe figure displays a sub-cellular layout of array peptides with phosphorylation levels significantly altered by lysates from human monocytes stimulated with N. meningitidis. IPA was used to identify transcriptional activity of these peptides (see Material and Methods for detailed algorithm). The kinase dual specificity tyrosine-(Y)-phosphorylation-regulated kinase 1A (DYRK1A) was identified to have transcriptional activity linked with the genes CDC6, CDK1, E2F1, E2F8, MCM3, MCM4, MYBL2, KNTC1, and UBEC2. The overlay function in IPA did not identify any of these genes to be regulated by N. meningitidis (green indicates down-regulation by N. meningitidis).
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cell functionsVEGFmonocyte model systemstudy changessignal transduction10 6 NPTENLarge-scale reductiontyrosine kinase activitiesgene expression changesIngenuity Pathway Analysiskinase activitycell lysatesNf -κBmeningococcal sepsistyrosine kinase-mediated signal transductionmRNA levelsIL -10 anti-inflammatory responsevivo array peptide phosphorylationmRNA datatumor necrosis factorarray peptide phosphorylationanti-inflammatory cytokine interleukin -10meningitidis Ncytokine release144 kinase peptide substratesCXCL 10.15 minutesTNFZBTB60 minutesSTAT 3 activitykinase activitieskinase substrate arrays
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