dNK cells and EVT cells identification.

Phenotypic analysis of isolated CD3⁻CD56⁺ human dNK cells and human extravillous trophoblast cells using flow cytometry and immunohistochemistry. (A) dNK cells were isolated from fresh human deciduas tissues and were stained using anti-CD3-FITC antibody and anti-CD56-PE-cy5 antibodies, and then analyzed by flow cytometry. The CD3⁻CD56⁺ dNK cells were about 98% pure. Human extravillous trophoblast cells were isolated from fresh villi tissues and were stained with anti-keratin-FITC antibody and anti-vimentin-PE antibody and then detected by immunohistochemistry. (B) Trophoblast cells stained for keratin. (C) Unstained trophoblasts.