brv mutations are novel alleles of Drosophila GlcAT-P.
(A, B) Phase contrast pictures of wild-type (brv+) and brv1 mutant L3 larva brains showing the elongation of the larval VNC in brv (B). Bar: 50 µm. (C) Graphical display of the deficiencies (Df) used in the complementation test in which the chromosomal region was narrowed to 60 kb. (+), (-) indicate complementing and non-complementing Df, respectively. (D) Illustration of the structure of the GlcAT-P locus flanked by the predicted genes CG14142 and CG6199. Arrows indicate the direction of transcription. Exons are boxed and translated regions are represented by filled boxes. The gray box indicates an exon which is spliced into one but not the other isoform. The positions in which the brv1, brv2 and brv3 alleles are mutated are indicated. (E) Analysis of GlcAT-P protein expression. Protein extracts from wild-type (brv+), Df(3L)GlcAT-P, brv1, brv2 and brv3 mutant L3 larvae were blotted with an anti-GlcAT-P antibody. Tub: α-tubulin loading control.
