Z-projections of confocal stacks of whole-mount brains double-stained with mAb nb168 and anti-PDH antiserum (A1-3) or mAb nb169 and anti-GFP in a fly expressing GFP driven by Janelia Gal4 line R49C04 (B1-3).
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posted on 2013-09-17, 01:58 authored by Beatriz Blanco Redondo, Melanie Bunz, Partho Halder, Madhumala K. Sadanandappa, Barbara Mühlbauer, Felix Erwin, Alois Hofbauer, Veronica Rodrigues, K. VijayRaghavan, Mani Ramaswami, Dirk Rieger, Christian Wegener, Charlotte Förster, Erich BuchnerThe perfect co-localization in A3 indicates that nb168 could recognize Drosophila PDF or its precursor protein, whereas co-localization in B3 is restricted to the two very large cells stained by nb169 in the posterior protocerebrum. Scale bar 100 µm.
