Western blotting (A) for G and M2-1 proteins, claudin-4, occludin, phospho-NF-κB and NF-κB, RT-PCR (B) for G protein, RIG-I and MDA5 and ELISA (C) for TNFα and RANTES in HNECs pretreated with 0.1–10 μg/ml MG132 before infection with RSV at an MOI of 1.
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posted on 2013-09-18, 01:40 authored by Kazufumi Obata, Takashi Kojima, Tomoyuki Masaki, Tamaki Okabayashi, Shinichi Yokota, Satoshi Hirakawa, Kazuaki Nomura, Akira Takasawa, Masaki Murata, Satoshi Tanaka, Jun Fuchimoto, Nobuhiro Fujii, Hiroyuki Tsutsumi, Tetsuo Himi, Norimasa SawadaThe corresponding expression levels are shown as error bar graphs. Data are means ± SEM. *p<0.05 versus only RSV. CLDN: claudin, OCLN: occludin.
