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Western blot analysis of G2/M arrest regulator proteins.

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posted on 2013-06-14, 00:34 authored by Birgit Lohberger, Beate Rinner, Nicole Stuendl, Heike Kaltenegger, Bibiane Steinecker-Frohnwieser, Eva Bernhart, Ehsan Bonyadi Rad, Annelie Martina Weinberg, Andreas Leithner, Rudolf Bauer, Nadine Kretschmer

Total protein analysis revealed a significant downregulation of cdc2 and cyclin B1 in dehydrocostus lactone treated STS cells, whereas, costunolide treatment did not affect the protein levels of these G2/M checkpoint regulators. β-actin was used as loading control. Data shown are representative from at least three independent experiments.

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