Western blot analysis of ER stress-related factors in STZ-treated RPTECs.
figure
posted on 2014-02-05, 04:07 authored by Ryuichi Uetake, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Hisaka Kawate, Yasuhiro Iesato, Takahiro Yoshizawa, Teruhide Koyama, Lei Yang, Yuichi Toriyama, Akihiro Yamauchi, Kyoko Igarashi, Megumu Tanaka, Takashige Kuwabara, Kiyoshi Mori, Motoko Yanagita, Masashi Mukoyama, Takayuki Shindo(A) Western blot analysis of ER stress sensor molecules in STZ-treated RPTECs. (B) Optical densities of Western blots were quantified, and the relative expression of BiP and ATF6 and the p-PERK/PERK and p-IRE1/IRE1 ratios (PERK and IRE1 activation level) are shown. (C) Western blot analysis of the mediators downstream of PERK. (D) Quantification of the results in (C). The experiments were repeated 3 times. Data from the vehicle group were assigned a value of 1. Bars are means ± SEM. *p<0.05, ***p<0.001.
