Western blot analyses of normal colon cell line FHC, primary cell line SW480 and HT-29 and metastatic SW620 and T84 for angiogenesis protein expression profiles.

Western analyses were performed with the normal cell line and primary and metastatic colorectal cancer cell lines. Angiogenesis proteins identified from the array study, coagulation factor III, IGFBP-3, DPP IV, PDGF-A, endothelin I and CXCL16 were run on the gel and analyzed. Protein bands were measured for intensity and quantified using normal colon cell FHC as a reference.