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Variations in the FRET response of FRET pairs on proteolytic cleavage over time.

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posted on 2015-08-03, 03:19 authored by Bobin George Abraham, Karen S. Sarkisyan, Alexander S. Mishin, Ville Santala, Nikolai V. Tkachenko, Matti Karp

The control is NowGFP-mRuby2 FRET pair without thrombin cleavage site (LVPS instead of LVPR). ΔR/R was computed as (R0-RF)/R0 where R is donor:acceptor ratio and R0 is the donor:acceptor ratio when there is no FRET and RF is the FRET ratio

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    FRET pairsProtein Variantsflimfluorescence lifetime measurementsF örster radiusfluorescence lifetime imaging microscopyFluorescence Lifetime Measurements Fluorescence Resonance Energy TransferFRET efficiency

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