posted on 2015-06-24, 03:54authored byJovan Rebolledo-Mendez, Matthew S. Hestand, Stephen J. Coleman, Zheng Zeng, Ludovic Orlando, James N. MacLeod, Ted Kalbfleisch
An example of three variant call format records. The first is a called heterozygote with an allele depth of 11, 6 reads containing the reference allele, and 5 reads containing the non-reference. The second, a called homozygote in the current analysis with an allele depth of 4, three high quality reads containing the non-reference allele and one low quality read containing the reference. The third, is an example of a called homozygote with an allele depth of 5, where all 5 reads contained the non-reference allele. Images of the reads used to derive the VCF records are shown within the IGV Browser. Base calls within the reads that agree with the reference are not rendered.