Validation of RNA sequencing by RT-qPCR.

Graphs showing fold changes between 4-cell/2-cell stages derived from ddCt values (black bars; qPCR) and identified by RNA sequencing (green bars; RNA-seq). Figure 1A shows the validation results for genes identified as enriched at 2-cell stage. Grey bars named Bc qPCR show fold changes between blastocyst/2-cell stages and demonstrate the tendency towards continuous decrease in the amount of these transcripts. Figure 1B shows the validation results for genes identified as enriched at 4-cell stage.