Uric acid up-regulates KHK in human hepatocytes.
figure
posted on 2012-10-24, 01:19 authored by Miguel A. Lanaspa, Laura G. Sanchez-Lozada, Christina Cicerchi, Nanxing Li, Carlos A. Roncal-Jimenez, Takuji Ishimoto, Myphuong Le, Gabriela E. Garcia, Jeffrey B. Thomas, Christopher J. Rivard, Ana Andres-Hernando, Brandi Hunter, George Schreiner, Bernardo Rodriguez-Iturbe, Yuri Y. Sautin, Richard J. Johnson(A–B) KHK protein expression in cells exposed to increasing levels of uric acid for 72 hours in the presence or absence of the URAT1 inhibitor probenecid (2 mmol/L). (C–D) KHK protein expression in cells exposed to 750 µmol/L uric acid for different time points in the presence or absence of the URAT1 inhibitor probenecid. (E–F) KHK protein expression in cells exposed to 5 mmol/L fructose for 72 hours in the presence or absence of the XO inhibitor allopurinol (100 µmol/L). G) KHK activity in cells exposed to 5 mmol/L fructose for 72 hours in the presence or absence of the XO inhibitor allopurinol (100 µmol/L).
