Urea production and cytochrome P450 activity of MPIO-labeled and unlabeled cells.
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posted on 2015-04-09, 03:39 authored by Dwayne R. Roach, Wesley M. Garrett, Glenn Welch, Thomas J. Caperna, Neil C. Talbot, Erik M. ShapiroA) Urea production with and without the addition of 5 μmol NH4Cl to the culture medium. B) P450 EROD activity after a 48-h 3-methylcholanthrine (5 μM) induction. Total fluorescent activity is with the addition of β-glucuronidase/arylsulfatase so as to detect total (left) and conjugated (right) EROD activity products. (n = 3, bars represent SEM) **P<0.01, ***P<0.001
