Trx2 overexpression and downregulation by in ovo electroporation.
figure
posted on 2015-11-05, 04:34 authored by Marc Pirson, Stéphanie Debrulle, André Clippe, Frédéric Clotman, Bernard Knoops(A-H) Immunofluorescence detection of Trx2 in E4.5 chick embryo spinal cords electroporated with Trx2 vector or MoTrx2. (I) Western blotting analysis of the contralateral (non-electroporated; left well) and ipsilateral (electroporated; right well) sides of dissected spinal cords of E4.5 chick embryos electroporated with empty vector, Trx2 vector, MoCTRL or MoTrx2. (J) Densitometric analysis of Trx2 expression, shown as the ratio of the ipsilateral measurement on the contralateral measurement. Scale bar = 50 μm.
