Transplantation of ESC-derived FLSs under the kidney capsule of SCID mice.

(A) Transplantation of newborn mouse ovary under the kidney capsule of a SCID mouse. Arrowhead indicates the grafted ovary 2 weeks after transplantation. (B) PAS staining of neonatal ovary sections before transplantation. No antral follicles were observed. Scale bar, 100 μm. (C) PAS staining of the grafted ovary sections. The progression of folliculogenesis was observed in the graft as shown by antral cavity formation (arrowhead). Scale bar, 100 μm. (D) Teratoma formation following transplantation of clone-G ESC-derived FLSs under the kidney capsule of SCID mouse. (E) Sections of the teratoma derived from transplanted FLSs. The representative images show sections with gastrointestinal cells, cardiac muscle cells, muscle cells and cells that resembled pancreatic cells or glandula mammaria. Scale bar, 100 μm.