The single cell gel electrophoresis (SCGE) assay was used to evaluate DNA damage in L56Br-C1 cells.

Twenty-four h after seeding of L56Br-C1 cells, NSpd, Pd-NSpd or Pt-NSpd was added to give a final concentration of 25 µM. After 72 h of treatment, cells were harvested for SCGE analysis. The ethidium bromide-stained nucleoids were photographed and then examined using the Comet Score™ Freeware. A. Images of comets obtained by the SCGE assay. DNA damage results in comets with head and tail, whereas undamaged DNA results in a round head. B. Percentage DNA in tail on the x-axis versus tail length on the y-axis for individual cells. C. Tail moment TMOM (%DNA in tail multiplied by tail length) for individual cells. Data were collected from three independent experiments, n = 207 cells. D. Table showing the mean TMOM value of the 10% highest TMOM values i.e. 20 highest values ± SD. *p<0.05 compared to control; ***p<0.001 compared to control.